Fig. 6. SERT KO mice have impaired AhR activation in response to exogenously administered AhR ligands. (A-C) WT and SERT KO mice were administered β-naphthoflavone (β-NF) by gavage (50 mg/kg) in corn oil once a day for 4 days. Total RNA was isolated from jejunal (A), ileal (C), or colonic (C) mucosal scrapings. Data represent the relative expression of Cyp1a1 mRNA quantified by qPCR (n = 4-6). (D-H) WT and SERT KO mice were placed on an AIN-76A synthetic diet with or without 200 ppm indole-3-carbinol (I3C) for 3 weeks. Total RNA was isolated from jejunal (D), ileal (E), or colonic (F) mucosal scrapings as well as whole liver (G) and lung (H). Data represent the relative expression of Cyp1a1 mRNA quantified by qPCR (n = 6-12). Data analyzed by 2-Way ANOVA followed by Tukey's multiple comparison's test. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 between groups as indicated. n.s. = not significant.